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SOD2 Monoklonaler Antikörper

SOD2 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(67)

CloneNo.

3A6C2

Kat-Nr. : 66474-1-Ig

Synonyme

SODM, 3A6C2, EC:1.15.1.1, IPO B, IPOB



Geprüfte Anwendungen

Erfolgreiche Detektion in WBU2OS-Zellen, HeLa-Zellen, Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe, RAW 264.7-Zellen, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lebergewebe, humanes Pankreaskarzinomgewebe, Mauslebergewebe, Rattenlebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHUVEC-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66474-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA SOD2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Ziege
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SOD2 fusion protein Ag21388
Vollständiger Name superoxide dismutase 2, mitochondrial
Berechnetes Molekulargewicht 25 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SOD2 antibody 66474-1-IgProtokoll herunterladen
IHC protocol for SOD2 antibody 66474-1-IgProtokoll herunterladenl
IF protocol for SOD2 antibody 66474-1-IgProtokoll herunterladen
FC protocol for SOD2 antibody 66474-1-IgDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy

Authors - Lihao Jin
humanWB

Int J Biol Macromol

Ultra-small platinum nano-enzymatic spray with ROS scavenging and anti-inflammatory properties for photoaging treatment

Authors - Chunying Li
mouseWB,IF

J Control Release

A cerium oxide loaded glycol chitosan nano-system for the treatment of dry eye disease.

Authors - Fan Yu
mouseWB

Stem Cell Res Ther

Apoptotic vesicles resist oxidative damage in noise-induced hearing loss through activation of FOXO3a-SOD2 pathway

Authors - Xiaotong Huang
humanWB

Oxid Med Cell Longev

HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation.

Authors - Shangting Han
mouseWB

Mol Ther Nucleic Acids

Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis

Authors - Ni Zeng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Iru (Verified Customer) (07-30-2020)

This antibody works very well at 1:2000 dilution with O/N incubation.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fallopian tube epithelial cells
SOD2 Antibody Western Blot, validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:66474-1-Ig)