SOD2 Monoklonaler Antikörper

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• KD/KO Validated

SOD2 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(2)

CloneNo.

3A6C2

Kat-Nr. : CL594-66474

Synonyme

SODM, MNSOD, Mn SOD, IPOB, IPO B

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  IF Staining of HUVEC using CL594-66474

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using CL594-66474 (SOD2 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL594-66474

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human SOD2 (CL594-66474, Clone:3A6C2) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

CL594-66474 bindet in IF/ICC, FC (Intra) SOD2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SOD2 fusion protein Ag21388
• Vollständiger Name: superoxide dismutase 2, mitochondrial
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC016934
• Gene symbol: SOD2
• Gene ID (NCBI): 6648
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Publikationen

Species	Application	Title
human	IF,FC	EBioMedicine; Single-nucleus RNA sequencing reveals heterogenous microenvironments and specific drug response between cervical squamous cell carcinoma and adenocarcinoma; Authors - Shitong Lin
human	IF	Cell Death Dis; Single-cell transcriptome profiles the heterogeneity of tumor cells and microenvironments for different pathological endometrial cancer and identifies specific sensitive drugs; Authors - Fang Ren