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TDP-43 Polyklonaler Antikörper
TDP-43 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte, Zebrafisch und mehr (4)
Anwendung
WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM
Konjugation
Unkonjugiert
Kat-Nr. : 10782-2-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | SH-SY5Y-Zellen, C2C12-Zellen, HeLa-Zellen, Neuro-2a-Zellen |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Gliomgewebe, humanes Hirngewebe (FTLD-U), humanes Hirngewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-Fro | Rattenhirngewebe, Maushirngewebe |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Erfolgreiche ChIP | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:2000-1:8000 |
Immunfluoreszenz (IF)-FRO | IF-FRO : 1:1000-1:4000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:3000-1:12000 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
CHImmunpräzipitation (IP) | CHIP : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
Produktinformation
10782-2-AP bindet in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM TDP-43 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch
Getestete Reaktivität | human, Maus, Ratte, Zebrafisch |
In Publikationen genannte Reaktivität | human, Affe, hamster, Hefe, Huhn, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Fusionsprotein |
Vollständiger Name | TAR DNA binding protein |
Berechnetes Molekulargewicht | 43 kDa |
Beobachtetes Molekulargewicht | 44 kDa |
GenBank-Zugangsnummer | BC001487 |
Gene symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for TDP-43 antibody 10782-2-AP | Protokoll herunterladen |
IHC protocol for TDP-43 antibody 10782-2-AP | Protokoll herunterladenl |
IF protocol for TDP-43 antibody 10782-2-AP | Protokoll herunterladen |
IP protocol for TDP-43 antibody 10782-2-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Lancet Neurol A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study. | ||
Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Emilie (Verified Customer) (09-24-2025) | I used it at 1:1000 for WB and at 1:200 for IF, and it worked as expected.
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FH Manon (Verified Customer) (09-23-2025) | The antibody shows very nice staining by IF and also works well by WB
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FH Nikita (Verified Customer) (08-14-2025) | Used this for checking our in house TDP production quality on WB. We did a lot of tests with other Ab and we trust this one here.
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FH Cynthia (Verified Customer) (07-28-2025) | This antibody worked well for our lab.
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FH Paloma (Verified Customer) (03-13-2025) | We love the results of this antibody
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FH Jose (Verified Customer) (02-24-2025) | N/A
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FH Kevin (Verified Customer) (01-31-2025) | Worked well for Opal staining of TDP-43 in human brain tissue sections and visualization on confocal
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FH Scott (Verified Customer) (10-22-2024) | 10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size but with two bands, blue bars, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.
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FH Makenna (Verified Customer) (07-16-2024) | Didn't work well for western blot, had high background
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FH Paloma (Verified Customer) (03-26-2024) | We love this item since we start to use it.
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FH MANOHAR (Verified Customer) (03-06-2024) |
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FH David (Verified Customer) (01-02-2024) | Top notch antibody, single band at predicted molecular weight. Gold standard.
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FH Parijat (Verified Customer) (09-09-2023) | Good antibody for IF
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FH Florencia (Verified Customer) (07-17-2023) | This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.
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FH Pauline (Verified Customer) (02-23-2022) | everything ok
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FH Xin (Verified Customer) (01-23-2022) | Very good in WB with a band of around 45 kD.
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FH Jessica (Verified Customer) (06-10-2021) | good for WB and IF
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FH Ana (Verified Customer) (03-14-2021) | TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.
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FH Yasuyo (Verified Customer) (01-20-2021) | Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.
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FH MEIMEI (Verified Customer) (11-12-2020) | the antibody work prefect for my staining.
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FH Stephane (Verified Customer) (10-31-2020) | Good product for staining.
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FH Marina (Verified Customer) (10-25-2020) | TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.
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FH Uxoa (Verified Customer) (02-18-2020) | Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.
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FH Paul (Verified Customer) (01-15-2020) | Produces good IF images with minimal background.
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FH Laura (Verified Customer) (01-10-2020) | Works very well
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FH Benjamin (Verified Customer) (01-07-2020) | Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.
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FH Apoorva (Verified Customer) (12-18-2019) | It has great specificity over wide range of species.
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FH Tilly (Verified Customer) (11-27-2019) | Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.
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FH Azita (Verified Customer) (10-04-2019) | I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.
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FH Francisco (Verified Customer) (09-28-2019) | Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.
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FH Alinda (Verified Customer) (09-11-2019) | Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.
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FH David (Verified Customer) (07-18-2019) | Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.
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FH George (Verified Customer) (07-07-2019) | Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.
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FH Elena (Verified Customer) (08-17-2018) |
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FH Petra (Verified Customer) (03-06-2018) |
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