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TDP-43 Polyklonaler Antikörper

TDP-43 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, ChIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte, Zebrafisch und mehr (4)

Anwendung

WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM

Konjugation

Unkonjugiert

Publikationen(1608)

Kat-Nr. : 10782-2-AP

Synonyme

TDP-43 (N-terminal), TARDBP, TDP 43, TDP43, ALS10



Geprüfte Anwendungen

Erfolgreiche Detektion in WBSH-SY5Y-Zellen, C2C12-Zellen, HeLa-Zellen, Neuro-2a-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Gliomgewebe, humanes Hirngewebe (FTLD-U), humanes Hirngewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-FroRattenhirngewebe, Maushirngewebe
Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen
Erfolgreiche ChIPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:2000-1:8000
Immunfluoreszenz (IF)-FROIF-FRO : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:3000-1:12000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
CHImmunpräzipitation (IP)CHIP : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10782-2-AP bindet in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, ChIP, RIP, ELISA, IEM TDP-43 und zeigt Reaktivität mit human, Maus, Ratten, Zebrafisch

Getestete Reaktivität human, Maus, Ratte, Zebrafisch
In Publikationen genannte Reaktivitäthuman, Affe, hamster, Hefe, Huhn, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name TAR DNA binding protein
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 44 kDa
GenBank-ZugangsnummerBC001487
Gene symbol TDP-43
Gene ID (NCBI) 23435
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

 The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 10782-2-AP antibody is a rabbit polyclonal antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for TDP-43 antibody 10782-2-APProtokoll herunterladen
IHC protocol for TDP-43 antibody 10782-2-APProtokoll herunterladenl
IF protocol for TDP-43 antibody 10782-2-APProtokoll herunterladen
IP protocol for TDP-43 antibody 10782-2-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nature

Oxidative cyclization reagents reveal tryptophan cation-π interactions

Authors - Xiao Xie
mouseIF

Cell

Mouse oocytes sequester aggregated proteins in degradative super-organelles

Authors - Gabriele Zaffagnini
humanWB

Lancet Neurol

A C9orf72 promoter repeat expansion in a Flanders-Belgian cohort with disorders of the frontotemporal lobar degeneration-amyotrophic lateral sclerosis spectrum: a gene identification study.

Authors - Ilse Gijselinck
mouseIF

Cell Res

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Authors - Liang Guo
humanIF

Nature

TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A.

Authors - X Rosa Ma
mouseIF

Nature

Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.

Authors - Jiasheng Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Emilie (Verified Customer) (09-24-2025)

I used it at 1:1000 for WB and at 1:200 for IF, and it worked as expected.

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: IPSCs derived motoneurons
FH

Manon (Verified Customer) (09-23-2025)

The antibody shows very nice staining by IF and also works well by WB

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: iPSC-derived motor neurons
FH

Nikita (Verified Customer) (08-14-2025)

Used this for checking our in house TDP production quality on WB. We did a lot of tests with other Ab and we trust this one here.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse
FH

Cynthia (Verified Customer) (07-28-2025)

This antibody worked well for our lab.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
FH

Paloma (Verified Customer) (03-13-2025)

We love the results of this antibody

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: TDP43
  • Cell Tissue Type: Brain
FH

Jose (Verified Customer) (02-24-2025)

N/A

  • Applications: Cell culture
FH

Kevin (Verified Customer) (01-31-2025)

Worked well for Opal staining of TDP-43 in human brain tissue sections and visualization on confocal

  • Applications: Immunohistochemistry
  • Cell Tissue Type: Brain section
FH

Scott (Verified Customer) (10-22-2024)

10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size but with two bands, blue bars, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: TDP43 Q331K Mouse Brain Lysate
TDP-43 Antibody Western Blot validation (1:2000 dilution) in TDP43 Q331K Mouse Brain Lysate (Cat no:10782-2-AP)
FH

Makenna (Verified Customer) (07-16-2024)

Didn't work well for western blot, had high background

  • Applications: Western Blot
FH

Paloma (Verified Customer) (03-26-2024)

We love this item since we start to use it.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:2000-1:8000
  • Cell Tissue Type: IHQ for neuroscience
FH

MANOHAR (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue
FH

David (Verified Customer) (01-02-2024)

Top notch antibody, single band at predicted molecular weight. Gold standard.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y lysate
FH

Parijat (Verified Customer) (09-09-2023)

Good antibody for IF

  • Applications: Immunofluorescence
TDP-43 Antibody Immunofluorescence validation ( dilution) in (Cat no:10782-2-AP)
FH

Florencia (Verified Customer) (07-17-2023)

This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: iPSC-derived neurons
FH

Pauline (Verified Customer) (02-23-2022)

everything ok

  • Applications: Immunofluorescence
  • Cell Tissue Type: HeLa
FH

Xin (Verified Customer) (01-23-2022)

Very good in WB with a band of around 45 kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: mouse brain lysate
FH

Jessica (Verified Customer) (06-10-2021)

good for WB and IF

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:1000
FH

Ana (Verified Customer) (03-14-2021)

TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary human fibrosblasts
TDP-43 Antibody Western Blot validation (1:1000 dilution) in Primary human fibrosblasts (Cat no:10782-2-AP)
FH

Yasuyo (Verified Customer) (01-20-2021)

Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: fibroblast cell
FH

MEIMEI (Verified Customer) (11-12-2020)

the antibody work prefect for my staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: iPSCs
FH

Stephane (Verified Customer) (10-31-2020)

Good product for staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Neuron
TDP-43 Antibody Immunofluorescence validation (1:100 dilution) in Neuron (Cat no:10782-2-AP)
FH

Marina (Verified Customer) (10-25-2020)

TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
TDP-43 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:10782-2-AP)
FH

Uxoa (Verified Customer) (02-18-2020)

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human fibroblasts
TDP-43 Antibody Immunofluorescence, validation (1:100 dilution) in Human fibroblasts (Cat no:10782-2-AP)
FH

Paul (Verified Customer) (01-15-2020)

Produces good IF images with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: HEK293T
FH

Laura (Verified Customer) (01-10-2020)

Works very well

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
FH

Benjamin (Verified Customer) (01-07-2020)

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:2500, 1:100
  • Cell Tissue Type: HEK293T
FH

Apoorva (Verified Customer) (12-18-2019)

It has great specificity over wide range of species.

  • Applications: Western Blot,
FH

Tilly (Verified Customer) (11-27-2019)

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
  • Cell Tissue Type: Mouse brain tissue
FH

Azita (Verified Customer) (10-04-2019)

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
  • Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)
FH

Francisco (Verified Customer) (09-28-2019)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse
FH

Alinda (Verified Customer) (09-11-2019)

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SHSY5Y
TDP-43 Antibody Western Blot, validation (1:1000 dilution) in SHSY5Y (Cat no:10782-2-AP)
FH

David (Verified Customer) (07-18-2019)

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
  • Cell Tissue Type: Neuroblastoma, iPSC-derived neurons
FH

George (Verified Customer) (07-07-2019)

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Spinal Cord/Brain slices
FH

Elena (Verified Customer) (08-17-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK-293 cells
FH

Petra (Verified Customer) (03-06-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue