VDAC1/Porin Monoklonaler Antikörper

VDAC1/Porin Monoklonal Antikörper für WB, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(40)

CloneNo.

1E2C7

Kat-Nr. : 66345-1-Ig

Synonyme

Porin, VDAC1, Porin 31HL, PORIN 31 HL, Plasmalemmal porin


Filter:
  • Western Blot (WB) analysis of various lysates using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    WB analysis using 66345-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66345-1-Ig (VDAC1/Porin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    IF Staining of mouse liver using 66345-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using VDAC1/Porin antibody (66345-1-Ig, Clone: 1E2C7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HepG2 cells using VDAC1/Porin Monoclonal antibody (66345-1-Ig)

    FC experiment of HepG2 using 66345-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug VDAC1/Porin Monoclonal antibody (66345-1-Ig, Clone:1E2C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG3 isotype control Mouse McAb (66360-4-Ig, Clone: 1H4A5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

"VDAC1/Porin Antibodies" Comparison

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Geprüfte Anwendungen

Erfolgreiche Detektion in WBMDA-MB-231-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, RAW264.7-Zellen
Erfolgreiche Detektion in IF-PMauslebergewebe
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66345-1-Ig bindet in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA VDAC1/Porin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Maus / IgG3
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name voltage-dependent anion channel 1
Berechnetes Molekulargewicht 31 kDa
Beobachtetes Molekulargewicht 35-37 kDa
GenBank-ZugangsnummerNM_003374
Gene symbol Porin
Gene ID (NCBI) 7416
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for VDAC1/Porin antibody 66345-1-IgProtokoll herunterladen
IF protocol for VDAC1/Porin antibody 66345-1-IgProtokoll herunterladen
FC protocol for VDAC1/Porin antibody 66345-1-IgDownload protocol
STANDARD-PROTOKOLLE
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Publikationen

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu
WB

Proc Natl Acad Sci U S A

Ferroptosis as a target for protection against cardiomyopathy.

Authors - Xuexian Fang
humanWB

Free Radic Biol Med

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Authors - Zhiguo Li
mouse

Diabetes

PACS-2 Ameliorates Tubular Injury by Facilitating Endoplasmic Reticulum-Mitochondria Contact and Mitophagy in Diabetic Nephropathy.

Authors - Chenrui Li
mouseWB

Oxid Med Cell Longev

The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury.

Authors - Wei Tang
mouseWB

Cell Death Discov

Idebenone improves motor dysfunction, learning and memory by regulating mitophagy in MPTP-treated mice.

Authors - Junqiang Yan