ZW10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZW10 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 24561-1-AP

Synonyme

HZW10

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  WB analysis using 24561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24561-1-AP

  WB result of ZW10 antibody (24561-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZW10 transfected HeLa cells.

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  WB analysis of HeLa using 24561-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 24561-1-AP

  IP result of anti-ZW10 (IP:24561-1-AP, 4ug; Detection:24561-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IF Staining of HeLa using 24561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZW10 antibody (24561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 24561-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZW10 (24561-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, K-562-Zellen, Maushodengewebe, Rattenlebergewebe, Rattenhodengewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

24561-1-AP bindet in WB, IF/ICC, FC (Intra), IP, ELISA ZW10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZW10 fusion protein Ag19138
• Vollständiger Name: ZW10, kinetochore associated, homolog (Drosophila)
• Berechnetes Molekulargewicht: 779 aa, 89 kDa
• Beobachtetes Molekulargewicht: 88-90 kDa
• GenBank-Zugangsnummer: BC128264
• Gene symbol: ZW10
• Gene ID (NCBI): 9183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).

Publikationen

Species	Application	Title
human	WB,IF	J Clin Invest; RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia; Authors - Nathalie Launay
human	IF	Nat Commun; Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.; Authors - Haining Zhou
human	WB	Genes Dev; Functional dissection of human mitotic genes using CRISPR-Cas9 tiling screens.; Authors - Jacob A Herman
mouse	WB	J Cell Biol; Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions.; Authors - Dijin Xu
human,monkey	WB	Cell Rep; The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.; Authors - Dongfang Li ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk