c-SRC Monoklonaler Antikörper

c-SRC Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

hamster, human, Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen

1

CloneNo.

5E10C4

Kat-Nr. : CL594-60315

Synonyme

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1

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Galerie der Validierungsdaten

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  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL594-60315 (c-SRC antibody) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL594-60315

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594-conjugated c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-60315

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 c-SRC antibody (CL594-60315, Clone: 5E10C4 ) at dilution of 1:200.

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  FC experiment of MCF-7 using CL594-60315

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human c-SRC (CL594-60315, Clone:5E10C4) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MCF-7-Zellen, humanes Mammakarzinomgewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL594-60315 bindet in IF, FC (Intra) c-SRC und zeigt Reaktivität mit hamster, human, Maus

• Getestete Reaktivität: hamster, human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: c-SRC fusion protein Ag22306
• Vollständiger Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC011566
• Gene symbol: SRC
• Gene ID (NCBI): 6714
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

Publikationen

Species	Application	Title
human	IF	Mol Cancer; Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression.; Authors - Zhenzhen Pan