Alpha smooth muscle actin specific Monoklonaler Antikörper

Alpha smooth muscle actin specific Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(4)

CloneNo.

1E9A11

Kat-Nr. : CL647-67735

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form



Geprüfte Anwendungen

Erfolgreiche Detektion in IF-PMausherzgewebe
Erfolgreiche Detektion in FC (Intra)C2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 2 publications below
FCSee 1 publications below

Produktinformation

CL647-67735 bindet in IF-P, FC (Intra) Alpha smooth muscle actin specific und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name actin, alpha 2, smooth muscle, aorta
Berechnetes Molekulargewicht 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerNM_001613
Gene symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 647 Alpha smooth muscle actin specific antibody CL647-67735Protokoll herunterladen
FC protocol for CL Plus 647 Alpha smooth muscle actin specific antibody CL647-67735Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

J Med Virol

Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis

Authors - Arun Das
humanIF

Lab Invest

HSPB6 deficiency promotes the development of aortic dissection and rupture

Authors - Shiqi Gao
human

Adv Sci (Weinh)

Patient-Derived Tumor Organoids Combined with Function-Associated ScRNA-Seq for Dissecting the Local Immune Response of Lung Cancer

Authors - Chang Liu
mouseFC

Nat Commun

FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination

Authors - Chuxiang Lei