Alpha smooth muscle actin specific Monoklonaler Antikörper
Alpha smooth muscle actin specific Monoklonal Antikörper für IF-P, FC (Intra)
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte
Anwendung
IF-P, FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1E9A11
Kat-Nr. : CL647-67735
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in IF-P | Mausherzgewebe |
Erfolgreiche Detektion in FC (Intra) | C2C12-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
IF | See 2 publications below |
FC | See 1 publications below |
Produktinformation
CL647-67735 bindet in IF-P, FC (Intra) Alpha smooth muscle actin specific und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | actin, alpha 2, smooth muscle, aorta |
Berechnetes Molekulargewicht | 42 kDa |
Beobachtetes Molekulargewicht | 42 kDa |
GenBank-Zugangsnummer | NM_001613 |
Gene symbol | Alpha smooth muscle actin |
Gene ID (NCBI) | 59 |
Konjugation | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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IF protocol for CL Plus 647 Alpha smooth muscle actin specific antibody CL647-67735 | Protokoll herunterladen |
FC protocol for CL Plus 647 Alpha smooth muscle actin specific antibody CL647-67735 | Download protocol |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Med Virol Molecular and immune signatures, and pathological trajectories of fatal COVID-19 lungs defined by in situ spatial single-cell transcriptome analysis | ||
Adv Sci (Weinh) Patient-Derived Tumor Organoids Combined with Function-Associated ScRNA-Seq for Dissecting the Local Immune Response of Lung Cancer | ||
Nat Commun FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination |