Anticorps Polyclonal de lapin anti-Alpha Actin

Alpha Actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(182)

Avis (1)

N° de cat : 23660-1-AP

Synonymes

ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle

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Galerie de données de validation

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  WB analysis using 23660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 23660-1-AP

  IP result of anti-Alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human colon using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human skeletal muscle using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IHC staining of human heart using 23660-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

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  IF Staining of mouse heart using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 23660-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using 23660-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Alpha Actin antibody (23660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of C2C12 using 23660-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IF/ICC: cellules H9C2,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 158 publications below
• IHC: See 4 publications below
• IF: See 20 publications below

Informations sur le produit

23660-1-AP cible Alpha Actin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Actin Protéine recombinante Ag19151
• Nom complet: actin, alpha 1, skeletal muscle
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 39-42 kDaa
• Numéro d’acquisition GenBank: BC012597
• Symbole du gène: ACTA1
• Identification du gène (NCBI): 58
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Metab; QDPR deficiency drives immune suppression in pancreatic cancer; Authors - Ji Liu
mouse,human	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
	WB	J Exp Med; Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance; Authors - Naijun Miao
mouse	WB	Acta Pharm Sin B; A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis.; Authors - Tailin He
		Redox Biol; Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis; Authors - Xiangqi Chen
mouse	WB	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Arun (Verified Customer) (07-28-2025)

Works great even wat higher dilutions 1:10000

• Applications: Western Blot

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