Anticorps Monoclonal anti-Alpha cardiac muscle actin specific

Alpha cardiac muscle actin specific Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

CloneNo.

1F2B9

Avis (1)

N° de cat : 66125-1-Ig

Synonymes

ACTC1-specific, ACTC1, Alpha actin, 1F2B9, ACTC

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Galerie de données de validation

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  WB analysis using 66125-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66125-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens).

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  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human heart using 66125-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of mouse heart using 66125-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66125-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

• 

  FC experiment of C2C12 using 66125-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, cœur humain et tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF-Fro: tissu cardiaque de souris,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:2500-1:10000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

66125-1-Ig cible Alpha cardiac muscle actin specific dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha, cardiac muscle 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_005159
• Symbole du gène: ACTC1
• Identification du gène (NCBI): 70
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.

Publications

Species	Application	Title
mouse	WB	Life Sci; Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function; Authors - Michael L Fitch
	WB	J Am Heart Assoc; Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion.; Authors - Sumei Cui
human	IHC	Front Mol Biosci; Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer.; Authors - Longyang Liu
human	WB	Immunol Res; The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.; Authors - Muyao Ye
rat	WB,IF	Eur J Pharmacol; Puerarin ameliorates myocardial remodeling of spontaneously hypertensive rats through inhibiting TRPC6-CaN-NFATc3 pathway; Authors - Jiang Yan
human	IF	Amyloid; The flutemetamol analogue cyano-flutemetamol detects myocardial AL and ATTR amyloid deposits: a post-mortem histofluorescence analysis; Authors - Eric E Abrahamson

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-03-2024)

Worked very well for us

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000/1:2000
• Cell Tissue Type: NRVCMs

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