Anticorps Monoclonal anti-Alpha cardiac muscle actin specific

Alpha cardiac muscle actin specific Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

CloneNo.

1F2B9

N° de cat : CL594-66125

Synonymes

ACTC1-specific, ACTC1, Alpha actin, 1F2B9, ACTC

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Galerie de données de validation

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  IF Staining of mouse heart using CL594-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

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  IF Staining of mouse heart using CL594-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL594-66125 (ACTC1-specific antibody) at dilution of 1:100.

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  FC experiment of C2C12 using CL594-66125

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ACTC1-specific (CL594-66125, Clone:1F2B9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu cardiaque de souris
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-66125 cible Alpha cardiac muscle actin specific dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha, cardiac muscle 1
• Masse moléculaire calculée: 42 kDa
• Numéro d’acquisition GenBank: NM_005159
• Symbole du gène: ACTC1
• Identification du gène (NCBI): 70
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Publications

Species	Application	Title
mouse	IF	J Am Heart Assoc; α Protein Kinase 3 Is Essential for Neonatal and Adult Cardiac Function; Authors - Wei Feng

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