Anticorps Polyclonal de lapin anti-ADAM12

• Phare
• Validé par KD/KO

ADAM12 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(43)

N° de cat : 14139-1-AP

Synonymes

ADAM 12, Disintegrin and metalloproteinase domain-containing protein 12, EC:3.4.24.-, MCMP, Meltrin-alpha

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Galerie de données de validation

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  WB analysis of mouse brain using 14139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14139-1-AP

  IP result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14139-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ADAM12 Polyclonal antibody (14139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules MCF-7, tissu cardiaque de souris, tissu cérébral de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 26 publications below
• IHC: See 16 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

14139-1-AP cible ADAM12 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM12 Protéine recombinante Ag5206
• Nom complet: ADAM metallopeptidase domain 12
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 90-100 kDa, 70-80 kDa
• Numéro d’acquisition GenBank: BC060804
• Symbole du gène: ADAM12
• Identification du gène (NCBI): 8038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Publications

Species	Application	Title
mouse,human	IF	Cell Stem Cell; Vaccination-based immunotherapy to target profibrotic cells in liver and lung; Authors - Michal Sobecki
human	IHC	J Cell Biol; Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.; Authors - Díaz Bego?a B
		Matrix Biol; Antibodies and methods for immunohistochemistry of extracellular matrix proteins.; Authors - Steffen Rickelt
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	WB,IF	Cell Death Differ; ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.; Authors - M Aghababaei
human	IHC	J Hematol Oncol; Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.; Authors - Kosuke Ogawa

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