Anticorps Polyclonal de lapin anti-ADRA1A-Specific

• Phare
• Validé par KD/KO

ADRA1A-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 19777-1-AP

Synonymes

ADRA1A, ADRA1C, Alpha-1A adrenergic receptor, Alpha-1A adrenoceptor, Alpha-1A adrenoreceptor

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Galerie de données de validation

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  WB analysis using 19777-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours without or with blocking peptide (1.4 ug peptide was used to block the binding of 1.0 ug antibody).

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  WB analysis of HeLa using 19777-1-AP

  WB result of ADRA1A-Specific antibody (19777-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRA1A-Specific transfected HeLa cells.

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  WB analysis using 19777-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19777-1-AP (ADRA1A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 19777-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRA1A-Specific Polyclonal antibody (19777-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

19777-1-AP cible ADRA1A-Specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: adrenergic, alpha-1A-, receptor
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_000680
• Symbole du gène: ADRA1A
• Identification du gène (NCBI): 148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.

Publications

Species	Application	Title
human		Eur J Immunol; Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cells; Authors - Martin Jürgens
rat	WB	iScience; Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology.; Authors - Miaomiao Zhu
human	IHC,IF	Pharmaceuticals (Basel); Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease.; Authors - Miles Frost
human	WB	Vascul Pharmacol; The ERK1/2 pathway participates in the upregulation of the expression of mesenteric artery α1 receptors by intravenous tail injections of mmLDL in mice.; Authors - Jun-Jie Wang
mouse	WB	Heliyon; Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway; Authors - Bin Liu
rat	WB	BMC Neurosci; Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor; Authors - Chaoyong Tian

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