Anticorps Polyclonal de lapin anti-APJ

• Phare
• Validé par KD/KO

APJ Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 20341-1-AP

Synonymes

AGTRL1, Angiotensin receptor like 1, apelin receptor, APJ, APJR, APLNR, G protein coupled receptor APJ

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 20341-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20341-1-AP (APJ antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  FC experiment of SH-SY5Y using 20341-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug APJ antibody (20341-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Informations sur le produit

20341-1-AP cible APJ dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: APJ Protéine recombinante Ag14138
• Nom complet: apelin receptor
• Masse moléculaire calculée: 380 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC032688
• Symbole du gène: APLNR
• Identification du gène (NCBI): 187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APJ, also named APLNR and AGTRL1, belongs to the G-protein coupled receptor 1 family. It is a receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. APJ is an alternative coreceptor with CD4 for HIV-1 infection. It may be involved in the development of AIDS dementia. APJ is expressed abundantly in the corpus callosum and spinal cord.

Publications

Species	Application	Title
mouse	IHC	Cell Metab; Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis.; Authors - Katerina Rohlenova
mouse	IF	Nat Commun; Molecular determinants of nephron vascular specialization in the kidney.; Authors - David M Barry
human	WB,IHC,IF	Oxid Med Cell Longev; Apelin/APJ-Manipulated CaMKK/AMPK/GSK3β Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps.; Authors - Zhi-Ling Lou
rat	WB	Free Radic Biol Med; Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway; Authors - Liang Chen
mouse	WB	EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat	WB	Stem Cell Res Ther; ELABELA ameliorates hypoxic/ischemic-induced bone mesenchymal stem cell apoptosis via alleviation of mitochondrial dysfunction and activation of PI3K/AKT and ERK1/2 pathways.; Authors - Jiaying Fu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicole (Verified Customer) (02-24-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK cells

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