Anticorps Monoclonal anti-APPL1

• Phare
• Validé par KD/KO

APPL1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B7B11

N° de cat : 68195-1-Ig

Synonymes

DIP13A, Dip13 alpha, DCC-interacting protein 13-alpha, APPL, Adapter protein containing PH domain, PTB domain and leucine zipper motif 1

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Galerie de données de validation

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  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68195-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 68195-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig heart using 68195-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68195-1-Ig (APPL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68195-1-Ig

  WB result of APPL1 antibody (68195-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

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  IHC staining of human colon cancer using 68195-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68195-1-Ig (APPL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 68195-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (68195-1-Ig, Clone: 1B7B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 68195-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (68195-1-Ig, Clone:1B7B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cardiaque de lapin, tissu cardiaque de porc, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68195-1-Ig cible APPL1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APPL1 Protéine recombinante Ag3334
• Nom complet: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Masse moléculaire calculée: 709 aa, 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC028599
• Symbole du gène: APPL1
• Identification du gène (NCBI): 26060
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

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