Anticorps Polyclonal de lapin anti-cIAP1

• Phare
• Validé par KD/KO

cIAP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 10022-1-AP

Synonymes

API1, BIRC2, MIHB, RNF48, Baculoviral IAP repeat-containing protein 2

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 10022-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10022-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10022-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10022-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10022-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10022-1-AP (cIAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10022-1-AP (cIAP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10022-1-AP (cIAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10022-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10022-1-AP (cIAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10022-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using cIAP1 antibody (10022-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10022-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10022-1-AP (cIAP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10022-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug cIAP1 Polyclonal antibody (10022-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules Jurkat, tissu cérébral de souris, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu pancréatique de souris, tissu pancréatique humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10022-1-AP cible cIAP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: cIAP1 Protéine recombinante Ag15203
• Nom complet: baculoviral IAP repeat-containing 2
• Masse moléculaire calculée: 618 aa, 70 kDa
• Poids moléculaire observé: 70 kDa, 55-60 kDa
• Numéro d’acquisition GenBank: BC016174
• Symbole du gène: cIAP1
• Identification du gène (NCBI): 329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460). This BIRC2 antibody (10022-1-AP) can bind both full length (70kd) and cleaved form (60kd) of the protein.

Publications

Species	Application	Title
zebrafish	WB,IF	Nat Genet; Birc2 (cIap1) regulates endothelial cell integrity and blood vessel homeostasis.; Authors - Santoro Massimo M MM ; KD Validated
human	IF	Mol Cell; Liquid phase separation of NEMO induced by polyubiquitin chains activates NF-κB.; Authors - Mingjian Du
human	WB	Mol Cell; A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation.; Authors - Michael Hinz ; KD Validated
mouse	WB	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
human	WB	Dev Cell; Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform.; Authors - Maria Zachari
mouse	WB	J Immunol; Differential TRAF3 utilization by a variant human CD40 receptor with enhanced signaling.; Authors - Peters Anna L AL

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (09-23-2024)

Excellent

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1000
• Cell Tissue Type: A549

cIAP1 Antibody Immunoprecipitation validation (1000 dilution) in A549 (Cat no:10022-1-AP)

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