Anticorps Polyclonal de lapin anti-MUC1/CA15-3 C-terminal

• Phare
• Validé par KD/KO

MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 23614-1-AP

Synonymes

CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin

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Galerie de données de validation

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  WB analysis using 23614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas cancer using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:200 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 23614-1-AP (MUC1/CA15-3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 23614-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human MUC1/CA15-3 C-terminal (23614-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules BxPC-3, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu d'amygdalite humain, tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

23614-1-AP cible MUC1/CA15-3 C-terminal dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MUC1/CA15-3 C-terminal Protéine recombinante Ag20366
• Nom complet: mucin 1, cell surface associated
• Masse moléculaire calculée: 1264 aa, 123 kDa
• Poids moléculaire observé: 23-28 kDa
• Numéro d’acquisition GenBank: BC120975
• Symbole du gène: MUC1
• Identification du gène (NCBI): 4582
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

Publications

Species	Application	Title
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao
human	WB	Cell Cycle; LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.; Authors - Yue Li
human	WB	Pathol Res Pract; MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma; Authors - Jian Liu ; KD Validated
human	WB,IHC	Cell Death Discov; Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.; Authors - Zongwen Liang
mouse	WB	Cell Death Discov; Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.; Authors - Wenwen Wang
human	WB,IHC	Front Oncol; A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma; Authors - Xinxing Wang

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