Anticorps Polyclonal de lapin anti-Calpastatin

Calpastatin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 12250-1-AP

Synonymes

CAST, Calpain inhibitor, Sperm BS-17 component

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Galerie de données de validation

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  WB analysis using 12250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12250-1-AP (Calpastatin antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12250-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12250-1-AP (Calpastatin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12250-1-AP (Calpastatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12250-1-AP

  IP result of anti-Calpastatin (IP:12250-1-AP, 4ug; Detection:12250-1-AP 1:1000) with K-562 cells lysate 4000ug.

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  IHC staining of human lung cancer using 12250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12250-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12250-1-AP (Calpastatin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12250-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Calpastatin antibody (12250-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 12250-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calpastatin (12250-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12250-1-AP cible Calpastatin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Calpastatin Protéine recombinante Ag2897
• Nom complet: calpastatin
• Masse moléculaire calculée: 667 aa, 72 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC013579
• Symbole du gène: Calpastatin
• Identification du gène (NCBI): 831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions.

Publications

Species	Application	Title
human	WB	J Proteome Res; Quantitative Proteomic Analysis Reveals that Transmissible Gastroenteritis Virus (TGEV) Activates JAK-STAT1 Signaling Pathway.; Authors - Kang An
rat	WB	Biomedicines; Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure.; Authors - Cheng-Wei Lu
rat	WB,IF	Ann Anat; Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.; Authors - Shuchao Wang
human	WB	Oncotarget; Decreased calpain activity in chronic myeloid leukemia impairs apoptosis by increasing survivin in myeloid progenitors and xiap1 in differentiating granulocytes.; Authors - Weiqi Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (03-12-2020)

strong and predicted band for western blot from heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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