Anticorps Polyclonal de lapin anti-CD68

CD68 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

1

N° de cat : CL594-25747

Synonymes

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Galerie de données de validation

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  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200, Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), Collagen Type III (N-terminal) antibody (22734-1-AP, green).

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  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

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  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

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  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

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  FC experiment of RAW 264.7 using CL594-25747

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD68 (CL594-25747) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu de côlon humain, tissu d'amygdalite humain
• Résultats positifs en cytométrie: cellules RAW 264.7

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-25747 cible CD68 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD68 Protéine recombinante Ag22815
• Nom complet: CD68 molecule
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC015557
• Symbole du gène: CD68
• Identification du gène (NCBI): 968
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Publications

Species	Application	Title
mouse	IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C