Anticorps Polyclonal de lapin anti-CORO1A

CORO1A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (2)

N° de cat : 17760-1-AP

Synonymes

CLABP, Clipin A, CLIPINA, Clipin-A, CORO1

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Galerie de données de validation

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  WB analysis of rat brain using 17760-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17760-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17760-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17760-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17760-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17760-1-AP

  IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:3000) with mouse brain tissue lysate 1840 ug.

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  IP experiment of mouse spleen using 17760-1-AP

  IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.

• 

  IHC staining of mouse spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 17760-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 17760-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 17760-1-AP

  Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Jurkat using 17760-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HL-60, cellules Jurkat, tissu cérébral de rat, tissu de thymus de rat, tissu de thymus de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique de rat, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris, tissu de côlon de souris
• Résultats positifs en IF-Fro: tissu splénique de souris,
• Résultats positifs en FC (Intra): cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17760-1-AP cible CORO1A dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CORO1A Protéine recombinante Ag12139
• Nom complet: coronin, actin binding protein, 1A
• Masse moléculaire calculée: 461 aa, 51 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC110374
• Symbole du gène: CORO1A
• Identification du gène (NCBI): 11151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

Publications

Species	Application	Title
		Br J Pharmacol; PMID: 27128203; Authors - Xianrong Zhang
human	WB	Int Immunopharmacol; Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.; Authors - Fuli Min
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
rat	WB	PLoS One; Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.; Authors - Feng Guo
human	IF	Theranostics; TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy; Authors - Wen-Jie Gu
mouse	WB	Vet Res; The tryptophan-aspartate (WD) repeat domain of bovine Coronin-1A promotes mycobacterial survival by inhibiting calcium signaling-mediated phagosome-lysosome fusion; Authors - Jing Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jordan (Verified Customer) (12-04-2024)

Antibody worked well for western blot at 1:1000 overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

FH

lucie (Verified Customer) (06-25-2024)

Anti-CORO1A working in immunofluorescence on neutrophils fixed with PFA 2%, at a dilution of 1/200 in BSA 1%.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Neutrophils

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