Anticorps Polyclonal de lapin anti-MTCO2

• Phare
• Validé par KD/KO

MTCO2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

103

Avis (2)

N° de cat : 55070-1-AP

Synonymes

COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2, mtcox2

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Galerie de données de validation

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  WB analysis using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of various samples using 55070-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

• 

  WB analysis using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 55070-1-AP

  IP result of anti-MTCO2(IP:55070-1-AP, 4ug; Detection:55070-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (55070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of A431 using 55070-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MTCO2 (55070-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7, échantillons divers, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 93 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

55070-1-AP cible MTCO2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome c oxidase II
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 23-26 kDa
• Numéro d’acquisition GenBank: YP_003024029
• Symbole du gène: COX2
• Identification du gène (NCBI): 4513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.

Publications

Species	Application	Title
mouse	IF	Cell; Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.; Authors - Paul K Ziegler
human	WB	Cell Metab; Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.; Authors - Ruchi Masand
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
mouse	WB	Cell Res; Active RNA interference in mitochondria.; Authors - Kuanxing Gao
human	WB	Mol Cell; Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.; Authors - Xiaoyu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562 and KCL22

FH

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C

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14865-1-AP

ATP5A1 Polyclonal antibody

14676-1-AP

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10993-1-AP

TOM70 Polyclonal antibody

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SDHB Polyclonal antibody

10620-1-AP