Anticorps Polyclonal de lapin anti-MTCO2

• Phare
• Validé par KD/KO

MTCO2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(155)

Avis (8)

N° de cat : 55070-1-AP

Synonymes

COX2, COII, COX 2, COXII, MT CO2

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Galerie de données de validation

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  WB analysis using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of various samples using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  WB analysis of 143B.TK.P0 using 55070-1-AP

  WB result of MTCO2 antibody (MTCO2; 1:2000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

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  IP experiment of HeLa using 55070-1-AP

  IP result of anti-MTCO2 (IP:55070-1-AP, 4ug; Detection:55070-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 55070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (55070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of A431 using 55070-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MTCO2 (55070-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7, échantillons divers
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 140 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55070-1-AP cible MTCO2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cytochrome c oxidase II
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 23-26 kDa
• Numéro d’acquisition GenBank: YP_003024029
• Symbole du gène: MTCO2
• Identification du gène (NCBI): 4513
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.

Publications

Species	Application	Title
mouse	IF	Cell; Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.; Authors - Paul K Ziegler
human	WB	Cell Metab; Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.; Authors - Ruchi Masand
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
mouse	WB	Cell Res; Active RNA interference in mitochondria.; Authors - Kuanxing Gao
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Matthieu (Verified Customer) (09-24-2025)

Band is clearly visible at the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human fibroblasts

FH

Manon (Verified Customer) (09-24-2025)

vey nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Fibroblasts

FH

Emilie (Verified Customer) (09-24-2025)

Bands appeared sharp and well defined under the chosen dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

FH

Vignesh (Verified Customer) (09-03-2025)

It's a good product with reduced or no nonspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

FH

Lenie (Verified Customer) (07-18-2025)

The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/2000 (WB) and 1/200 (IF)
• Cell Tissue Type: Zebrafish

MTCO2 Antibody Western Blot,Immunofluorescence validation (1/2000 (WB) and 1/200 (IF) dilution) in Zebrafish (Cat no:55070-1-AP)

FH

Baptiste (Verified Customer) (05-23-2025)

Working perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Lymphoblastoid

MTCO2 Antibody Western Blot validation (1:10000 dilution) in Lymphoblastoid (Cat no:55070-1-AP)

FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562 and KCL22

FH

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)

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