Anticorps Monoclonal anti-CREB3L1,OASIS

CREB3L1,OASIS Monoclonal Antibody for WB, ELISA, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA, FC (Intra)

Conjugaison

Non conjugué

CloneNo.

2B5B4

N° de cat : 67617-1-Ig

Synonymes

CREB3L1, OASIS, Old astrocyte specifically-induced substance, Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1

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Galerie de données de validation

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  WB analysis using 67617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67617-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67617-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using 67617-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules 4T1, cellules A375, cellules A549, cellules HeLa, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67617-1-Ig cible CREB3L1,OASIS dans les applications de WB, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CREB3L1,OASIS Protéine recombinante Ag28042
• Nom complet: cAMP responsive element binding protein 3-like 1
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC015781
• Symbole du gène: CREB3L1/OASIS
• Identification du gène (NCBI): 90993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

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