Anticorps Polyclonal de lapin anti-CROT

• Phare
• Validé par KD/KO

CROT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 13543-1-AP

Synonymes

COT, EC:2.3.1.137, Peroxisomal carnitine O-octanoyltransferase

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Galerie de données de validation

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  WB analysis using 13543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13543-1-AP (CROT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13543-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13543-1-AP (CROT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13543-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13543-1-AP (CROT antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 13543-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CROT antibody (13543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 13543-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CROT Polyclonal antibody (13543-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13543-1-AP cible CROT dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CROT Protéine recombinante Ag4486
• Nom complet: carnitine O-octanoyltransferase
• Masse moléculaire calculée: 612 aa, 66 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC039004
• Symbole du gène: CROT
• Identification du gène (NCBI): 54677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CROT, also named as COT, belongs to the carnitine/choline acetyltransferase family. It is a beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. CROT converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CROT is an active forms for carnitine octanoyltransferase. This antibody can bind the close sequences genes.

Publications

Species	Application	Title
human	WB,IHC,IF	Front Endocrinol (Lausanne); The miR-33a-5p/CROT axis mediates ovarian cancer cell behaviors and chemoresistance via the regulation of the TGF-β signal pathway; Authors - Xin Li ; KD Validated
human	WB	J Biol Chem; Carnitine octanoyltransferase is important for the assimilation of exogenous acetyl-L-carnitine into acetyl-CoA in mammalian cells; Authors - Jake Hsu ; KD Validated
human	WB	J Biol Chem; Carnitine o-octanoyltransferase is a p53 target that promotes oxidative metabolism and cell survival following nutrient starvation; Authors - Jack D Sanford ; KD Validated
mouse	WB	Am J Transl Res; Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice; Authors - Wenci Weng
mouse	WB	iScience; Etomoxir repurposed as a promiscuous fatty acid mimetic chemoproteomic probe; Authors - Joseph Choi

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