Anticorps Recombinant de lapin anti-DRP1 (C-terminal)

• Phare
• Validé par KD/KO

DRP1 (C-terminal) Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2A19

N° de cat : 81561-1-RR

Synonymes

DRP1, 2A19, DLP1, DNM1L, DNM1L,DLP1

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Galerie de données de validation

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  WB analysis using 81561-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 81561-1-RR

  WB result of DRP1 (C-terminal) antibody (81561-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HEK-293 cells.

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  WB analysis using 81561-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-12 using 81561-1-RR

  IP result of anti-DRP1 (C-terminal) (IP:81561-1-RR, 4ug; Detection:81561-1-RR 1:5000) with PC-12 cells lysate 1560 ug.

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  IP experiment of rat brain using 81561-1-RR

  IP result of anti-DRP1 (C-terminal) (IP:81561-1-RR, 4ug; Detection:81561-1-RR 1:5000) with rat brain tissue lysate 1720 ug.

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  IHC staining of mouse cerebellum using 81561-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 81561-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 81561-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DRP1 (C-terminal) antibody (81561-1-RR, Clone: 2A19 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 81561-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (81561-1-RR, Clone:2A19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81561-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81561-1-RR against Human DRP1 (C-terminal) were performed. The affinity constant is 0.825 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HCT 116, cellules HEK-293, cellules NIH/3T3, cellules PC-12, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules PC-12, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81561-1-RR cible DRP1 (C-terminal) dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DRP1 (C-terminal) Protéine recombinante Ag3644
• Nom complet: dynamin 1-like
• Masse moléculaire calculée: 736 aa, 82 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC024590
• Symbole du gène: DRP1
• Identification du gène (NCBI): 10059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.

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