Anticorps Polyclonal de lapin anti-Desmin

Desmin Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-16520

Synonymes

CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

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Galerie de données de validation

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  IF Staining of mouse small intestine using CL488-16520

  Adult mouse small intestine was co-stained with desmin (CL488-16520, in cyan), E-cadherin (20874-1-AP, in red), and PCNA (60097-1-Ig, in green). Desmin marks the stromal cells and smooth muscle, E-cadherin marks the gut epithelial cells, and PCNA marks proliferating cells. The image was created in paid partnership with @Immunofluorescence on Instagram.

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  IF Staining of rat heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:100.

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  IF Staining of mouse heart using CL488-16520

  E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

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  IF Staining of mouse heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488-conjugated Desmin antibody (CL488-16520) at dilution of 1:100.

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  IF Staining of mouse heart using CL488-16520

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488-conjugated Desmin antibody (CL488-16520) at dilution of 1:100.

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  FC experiment of C2C12 using CL488-16520

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmin (CL488-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu d'intestin grêle de souris, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en cytométrie: cellules C2C12

Dilution recommandée

• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-16520 cible Desmin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Desmin Protéine recombinante Ag9742
• Nom complet: desmin
• Masse moléculaire calculée: 470 aa, 54 kDa
• Poids moléculaire observé: 52-53 kDa
• Numéro d’acquisition GenBank: BC032116
• Symbole du gène: DES
• Identification du gène (NCBI): 1674
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C