Anticorps Polyclonal de lapin anti-FOXO1

• Phare
• Validé par KD/KO

FOXO1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

113

Avis (2)

N° de cat : 18592-1-AP

Synonymes

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A

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Galerie de données de validation

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  WB analysis using 18592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18592-1-AP (FOXO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18592-1-AP

  IP result of anti-FOXO1(IP:18592-1-AP, 4ug; Detection:18592-1-AP 1:2000) with HepG2 cells lysate 960 ug.

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  IHC staining of human kidney using 18592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18592-1-AP (FOXO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FOXO1 antibody (18592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 18592-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FOXO1 (18592-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HEK-293T, cellules HepG2, cellules SGC-7901, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 103 publications below
• IHC: See 11 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below

Informations sur le produit

18592-1-AP cible FOXO1 dans les applications de WB, IP, IHC, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOXO1 Protéine recombinante Ag13296
• Nom complet: forkhead box O1
• Masse moléculaire calculée: 655 aa, 70 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC021981
• Symbole du gène: FOXO1
• Identification du gène (NCBI): 2308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

FOXO1, also named as FOXO1A, FKHR and FKH1, is a member of the FOXO subfamily of Forkhead transcription factors. FOXO1 is a transcription factor which acts as a regulator of cell responses to oxidative stress. FOXO1 interacts with LRPPRC and SIRT1. In the presence of KIRT1, FOXO1 mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. FOXO1 contains three predicted protein kinase B phosphorylation sites (Thr-24, Ser-256, and Ser-319) that are conserved in other FOXO proteins. The t(2;13) and the variant t(1;13) translocations generate PAX3/FKHR and PAX7/FKHR fusion proteins respectively. The resulting protein is a transcriptional activator. Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2).

Publications

Species	Application	Title
mouse	WB	J Exp Med; PD-1 and TIM-3 differentially regulate subsets of mouse IL-17A–producing γδ T cells; Authors - Sarah C Edwards
human	WB,ChIP	Cell Res; Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.; Authors - Yanjing Guo
mouse	WB	Redox Biol; miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation; Authors - Xiaofei Wang
mouse	WB,IF	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
mouse	IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou
mouse	WB	Small; Self-Therapeutic Nanoparticle That Alters Tau Protein and Ameliorates Tauopathy Toward a Functional Nanomedicine to Tackle Alzheimer's.; Authors - Sunil Kumar Vimal

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (01-27-2023)

an specific band is observed around 20 KDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/4000
• Cell Tissue Type: hepg2

FOXO1 Antibody Western Blot validation (1/4000 dilution) in hepg2 (Cat no:18592-1-AP)

FH

Ruchi (Verified Customer) (07-08-2022)

I used the free sample antibody for immunofluorescence and I am happy with the results. I would like to order Proteintech products for my lab needs

• Applications: Immunofluorescence
• Primary Antibody Dilution: FOXO1
• Cell Tissue Type: Mouse skin

FOXO1 Antibody Immunofluorescence validation (FOXO1 dilution) in Mouse skin (Cat no:18592-1-AP)

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