Anticorps Polyclonal de lapin anti-GABARAPL1-Specific

GABARAPL1-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 18721-1-AP

Synonymes

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GABARAPL1-Specific, GEC 1, GEC1

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Galerie de données de validation

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  WB analysis using 18721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 18721-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 18721-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18721-1-AP (GABARAPL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18721-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18721-1-AP (GABARAPL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 18721-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GABARAPL1 antibody (18721-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral de rat, tissu hépatique de souris, tissu rénal de souris, tissu splénique humain
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

18721-1-AP cible GABARAPL1-Specific dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GABA(A) receptor-associated protein like 1
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: NM_031412
• Symbole du gène: GABARAPL1
• Identification du gène (NCBI): 23710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. And at very low levels in thymus and small intestine.The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

Publications

Species	Application	Title
human	WB	Autophagy; GABARAPL1 antibodies: target one protein, get one free!; Authors - Le Grand Jaclyn Nicole JN
human	WB	Sci Rep; Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.; Authors - Alexandra Boeske
human	WB	BMC Musculoskelet Disord; MiR-145 inhibits the differentiation and proliferation of bone marrow stromal mesenchymal stem cells by GABARAPL1 in steroid-induced femoral head necrosis; Authors - Pengfei Xu