Anticorps Monoclonal anti-GEFT

GEFT Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

5A11F8

N° de cat : 66012-1-Ig

Synonymes

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 66012-1-Ig

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 66012-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66012-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66012-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66012-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66012-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 66012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 66012-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEFT antibody (66012-1-Ig, Clone: 5A11F8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HEK-293 using 66012-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66012-1-Ig cible GEFT dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: GEFT Protéine recombinante Ag10819
• Nom complet: RhoA/RAC/CDC42 exchange factor
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 53 kDa, 63 kDa
• Numéro d’acquisition GenBank: BC012860
• Symbole du gène: GEFT
• Identification du gène (NCBI): 115557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).

Publications

Species	Application	Title
human	WB	Cancer Sci; Inhibition of heat shock protein 90 destabilizes receptor tyrosine kinase ROR1 in lung adenocarcinoma.; Authors - Behnoush Khaledian

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