Anticorps Polyclonal de lapin anti-GPT2

• Phare
• Validé par KD/KO

GPT2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (Intra), FC, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 16757-1-AP

Synonymes

AAT2, Alanine aminotransferase 2, ALAT2, ALT2, GPT 2, GPT2

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Galerie de données de validation

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  WB analysis using 16757-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16757-1-AP

  WB result of GPT2 antibody (16757-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPT2 transfected HepG2 cells.

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  WB analysis of mouse kidney using 16757-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16757-1-AP (GPT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16757-1-AP

  IP Result of anti-GPT2 (IP:16757-1-AP, 4ug; Detection:16757-1-AP 1:500) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16757-1-AP (GPT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16757-1-AP (GPT2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16757-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT2 antibody (16757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 16757-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human GPT2 (16757-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

16757-1-AP cible GPT2 dans les applications de WB, IP, IHC, IF, FC (Intra), FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GPT2 Protéine recombinante Ag10273
• Nom complet: glutamic pyruvate transaminase (alanine aminotransferase) 2
• Masse moléculaire calculée: 523 aa, 58 kDa
• Poids moléculaire observé: 58 kDa, 47 kDa
• Numéro d’acquisition GenBank: BC062555
• Symbole du gène: GPT2
• Identification du gène (NCBI): 84706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GPT2 (Alanine aminotransferase 2; ALT2) is a key enzyme in intermediatary metabolism, catalysing the reversible transfer of the amino group of glutamate to pyruvate, yielding alanine and ketoglutarate. GPT2 protein localizes to mitochondria, and is mainly expressed in the pancreas, brain, adrenal gland, skeletal muscle and heart. Serum GPT2 is a useful marker for liver damage. Recently mutation of GPT2 has been reported to be a cause of neural disorder (27601654). 16757-1-AP antibody recognizes two different isoforms of GPT2 protein.

Publications

Species	Application	Title
	WB	Cell Metab; Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.; Authors - Qiuying Chen
mouse	WB	Cell Res; Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.; Authors - Wei-Feng Cai ; KD Validated
human	WB	Nat Commun; Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.; Authors - Yun-Qian Wang
human	WB	Cancer Res; Targeting BCAT1 combined with α-ketoglutarate triggers metabolic synthetic lethality in glioblastoma.; Authors - Bo Zhang
MOUSE	WB,IF	Proc Natl Acad Sci U S A; Mutations in mitochondrial enzyme GPT2 cause metabolic dysfunction and neurological disease with developmental and progressive features.; Authors - Qing Ouyang
human	IHC	Cell Rep; Thyroid hormone regulates glutamine metabolism and anaplerotic fluxes by inducing mitochondrial glutamate aminotransferase GPT2; Authors - Annunziata Gaetana Cicatiello

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