Anticorps Polyclonal de lapin anti-HRAS-Specific

• Phare
• Validé par KD/KO

HRAS-Specific Polyclonal Antibody for FC (Intra), IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

49

Avis (1)

N° de cat : 18295-1-AP

Synonymes

C BAS/HAS, C H RAS, C HA RAS1, CTLO, GTPase HRas, H Ras 1, H RASIDX, Ha Ras, HAMSV, HRAS, HRAS1, HRAS-Specific, K RAS, RASH1, Transforming protein p21

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Galerie de données de validation

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  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18295-1-AP

  WB result of HRAS-Specific antibody (18295-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRAS-Specific transfected HEK-293 cells.

• 

  WB analysis using 18295-1-AP

  Recombinant proteins (ag27444 (6*His tag-KRAS-2A), ag21645(6*His tag-KRAS-2B), ag29746(6*His tag-NRAS), ag23265(GST-HRAS)) were subjected to SDS PAGE followed by western blot with 16155-1-AP (KRAS-2B-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 18295-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18295-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18295-1-AP

  IP result of anti-HRAS-Specific(IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with mouse brain tissue lysate 1840 ug.

• 

  IP experiment of HEK-293 using 18295-1-AP

  IP result of anti-HRAS-Specific(IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:1000) with HEK-293 cells lysate 1480 ug.

• 

  IHC staining of human kidney using 18295-1-PBS

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 18295-1-AP (HRAS-Specific Antibody) at dilution of 1:50.

• 

  IF Staining of MCF-7 using 18295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HRAS-Specific antibody (18295-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 18295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human HRAS-Specific (18295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules C6, cellules NIH/3T3, cellules PC-3, cellules SH-SY5Y, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris, cellules HEK-293
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 43 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

18295-1-AP cible HRAS-Specific dans les applications de WB, IP, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-Ha-ras Harvey rat sarcoma viral oncogene homolog
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC006499
• Symbole du gène: HRAS
• Identification du gène (NCBI): 3265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HRAS, also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can't recognize NRAS and KRAS.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Targeting KRAS-dependent tumors with AZD4785, a high-affinity therapeutic antisense oligonucleotide inhibitor of KRAS.; Authors - Sarah J Ross
human	WB	Sci Transl Med; The ERBB network facilitates KRAS-driven lung tumorigenesis.; Authors - Björn Kruspig
human	IP	Mol Cell; KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis; Authors - Hiroshi Senoo
human	WB	J Exp Med; Genome-wide CRISPR/Cas9 screens reveal shared and cell-specific mechanisms of resistance to SHP2 inhibition; Authors - Wei Wei
human	IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
human	WB	Nat Commun; A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS.; Authors - Nicolas Bery

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Benjamin (Verified Customer) (01-10-2020)

Identified HRAS around 20kDa mark on western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK

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