Anticorps Polyclonal de lapin anti-KIF15

• Phare
• Validé par KD/KO

KIF15 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(30)

N° de cat : 55407-1-AP

Synonymes

hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15, KLP2

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Galerie de données de validation

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  WB analysis of HEK-293 using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HEK-293 cells.

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  WB analysis of Jurkat using 55407-1-AP

  WB result of KIF15 antibody (55407-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected Jurkat cells.

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  WB analysis using 55407-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 55407-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 55407-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55407-1-AP (KIF15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 55407-1-AP

  IP result of anti-KIF15 (IP:55407-1-AP, 4ug; Detection:55407-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 55407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55407-1-AP (KIF15 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HEK-293 using 55407-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug KIF15 Polyclonal antibody (55407-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules MDA-MB-453s, tissu de thymus de souris
• Résultats positifs en IP: tissu de thymus de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 28 publications below
• IHC: See 14 publications below
• IF: See 8 publications below

Informations sur le produit

55407-1-AP cible KIF15 dans les applications de WB, IHC, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: kinesin family member 15
• Masse moléculaire calculée: 160 kDa
• Poids moléculaire observé: 150-165 kDa
• Numéro d’acquisition GenBank: NM_020242
• Symbole du gène: KIF15
• Identification du gène (NCBI): 56992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med; Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis; Authors - David Zhang
human	WB,IHC	Cancer Res; KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.; Authors - Lin Gao ; KD Validated
human	IHC	Cancer Cell Int; Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer; Authors - Enkui Zhang
human	WB,IHC	Cell Physiol Biochem; Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.; Authors - Yuan Qiao ; KD Validated
mouse	WB	Front Cell Dev Biol; Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure.; Authors - Sai Aung Phyo
human	WB,IHC,IF	Transl Oncol; METTL3 methylated KIF15 promotes nasopharyngeal carcinoma progression and radiation resistance by blocking ATG7-mediated autophagy through the activation of STAT3 pathway; Authors - Siwei Li

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