Anticorps Monoclonal anti-Cytokeratin 19

• Phare
• Validé par KD/KO

Cytokeratin 19 Monoclonal Antibody for WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(16)

CloneNo.

3G1E4

Avis (1)

N° de cat : 60187-1-Ig

Synonymes

CK 19, CK19, KRT19, 3G1E4, CK-19

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Galerie de données de validation

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  WB analysis using 60187-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of MCF-7 using 60187-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60187-1-Ig

  WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

• 

  WB analysis of HaCaT using 60187-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60187-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:25000 (under 40x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:25000 (under 10x lens). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Staining was performed on the Epredia 480S Autostainer.

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60187-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:12800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse breast cancer using 60187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HeLa using 60187-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 19 (60187-1-Ig, Clone:3G1E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HaCaT, cellules HeLa, cellules PC-3, cellules T-47D, tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IHC-Autostainer: tissu de côlon humain,
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer de la thyroïde humain, tissu de cancer de l'estomac humain, tissu de cancer de l'œsophage humain, tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,
• Résultats positifs en IF-Fro: mouse breast cancer,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:12500-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:51200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 10 publications below

Informations sur le produit

60187-1-Ig cible Cytokeratin 19 dans les applications de WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cytokeratin 19 Protéine recombinante Ag7407
• Nom complet: keratin 19
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC002539
• Symbole du gène: Cytokeratin 19
• Identification du gène (NCBI): 3880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy; Authors - Xiaoyu Liu
human	WB	Stem Cells; Annexin A3 as a Potential Target for Immunotherapy of Liver Cancer Stem-Like Cells.; Authors - Qiu-Zhong Pan
human	IHC,IF	Stem Cell Res Ther; DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis; Authors - S Y Wang
human	IF	Breast Cancer Res; Single-cell RNA reveals a tumorigenic microenvironment in the interface zone of human breast tumors; Authors - Wei Yang
mouse	IF	Commun Biol; Chemerin attenuates acute kidney injury by inhibiting ferroptosis via the AMPK/NRF2/SLC7A11 axis; Authors - Yidan Ma
mouse	IF	Biomed Pharmacother; Serotonin-RhoA/ROCK axis promotes acinar-to-ductal metaplasia in caerulein-induced chronic pancreatitis.; Authors - Xufeng Tao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kis (Verified Customer) (02-21-2025)

This antibody is working. well for IF anlysis in human and mouse liver samples.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300

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