Anticorps Polyclonal de lapin anti-SLP76

SLP76 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12728-1-AP

Synonymes

LCP2, SLP-76 tyrosine phosphoprotein, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

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Galerie de données de validation

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  WB analysis using 12728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12728-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 12728-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12728-1-AP

  IP result of anti-SLP76 (IP:12728-1-AP, 4ug; Detection:12728-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human tonsillitis using 12728-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12728-1-AP (SLP76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12728-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12728-1-AP (SLP76 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 12728-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SLP76 antibody (12728-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of Jurkat using 12728-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SLP76 (12728-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, RAW264.7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Jurkat,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below

Informations sur le produit

12728-1-AP cible SLP76 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLP76 Protéine recombinante Ag3432
• Nom complet: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Masse moléculaire calculée: 533 aa, 60 kDa
• Poids moléculaire observé: 70-76 kDa
• Numéro d’acquisition GenBank: BC016618
• Symbole du gène: SLP76
• Identification du gène (NCBI): 3937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Front Immunol; Using weighted gene co-expression network analysis to identify key genes related to preeclampsia; Authors - Xinyang Shen
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
mouse	IHC	Front Genet; Identification of Key Genes and Potential New Biomarkers for Ovarian Aging: A Study Based on RNA-Sequencing Data.; Authors - Lingwei Ma
human	IHC	Medicine (Baltimore); Identification of key genes in salivary gland in Sjögren's syndrome complicated with Hashimoto thyroiditis: Common pathogenesis and potential diagnostic markers; Authors - Kaiyuan Zhang
human	WB	Bioorg Chem; Design, synthesis, and biological evaluation of novel HPK1 inhibitors possessing 3-cyano-quinoline moiety; Authors - Long Chen

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