Anticorps Monoclonal anti-LDHA

• Phare
• Validé par KD/KO

LDHA Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(32)

CloneNo.

2E2G6

N° de cat : 66287-1-Ig

Synonymes

LDH-A, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

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Galerie de données de validation

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  WB analysis using 66287-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66287-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66287-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66287-1-Ig (LDHA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66287-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66287-1-Ig (LDHA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66287-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66287-1-Ig(LDHA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66287-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA Monoclonal antibody (66287-1-Ig, Clone:2E2G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de porc, cellules A431, muscle squelettique de rat, muscle squelettique de souris, tissu de muscle squelettique de porc
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• IP: See 3 publications below

Informations sur le produit

66287-1-Ig cible LDHA dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LDHA Protéine recombinante Ag21417
• Nom complet: lactate dehydrogenase A
• Masse moléculaire calculée: 332 aa, 37 kDa
• Poids moléculaire observé: 32-40 kDa
• Numéro d’acquisition GenBank: BC067223
• Symbole du gène: LDHA
• Identification du gène (NCBI): 3939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies. Mutations in LDHA have been linked to exertional myoglobinuria. LDHA has some isoforms with MW 26-40 kDa.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
mouse	WB	Cell Metab; Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation; Authors - Huan Tong
mouse	WB,IF	Small; A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis; Authors - Jian Xie
human,mouse	WB,IHC,IP	Cell Death Dis; FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis; Authors - Changhao Wu ; KD Validated
mouse	WB	Life Sci; Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice; Authors - Lei Xu
rat	WB	J Cell Mol Med; PMID: 31945804; Authors - Chenyang Chen

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