Anticorps Monoclonal anti-MMP2

• Phare
• Validé par KD/KO

MMP2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(83)

CloneNo.

2B10D1

Avis (3)

N° de cat : 66366-1-Ig

Synonymes

2B10D1, 72 kDa gelatinase, 72 kDa type IV collagenase, CLG4A, EC:3.4.24.24

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Galerie de données de validation

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  WB analysis using 66366-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 66366-1-Ig

  WB result of MMP2 antibody (66366-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells.

• 

  WB analysis of human placenta using 66366-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66366-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66366-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66366-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66366-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66366-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66366-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 66366-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of PC-3 using 66366-1-Ig

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules MCF-7, cellules MG U-87, tissu cardiaque de porc, tissu cardiaque de rat, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 76 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Informations sur le produit

66366-1-Ig cible MMP2 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP2 Protéine recombinante Ag25039
• Nom complet: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 55-74 kDa
• Numéro d’acquisition GenBank: BC002576
• Symbole du gène: MMP2
• Identification du gène (NCBI): 4313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).

Publications

Species	Application	Title
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
human	WB	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
rat	WB,IF	J Neuroinflammation; SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury; Authors - Chenglong Wang
rat	WB	Int J Nanomedicine; A Novel Liposomal S-Propargyl-Cysteine: A Sustained Release of Hydrogen Sulfide Reducing Myocardial Fibrosis via TGF-β1/Smad Pathway.; Authors - Ba Hieu Tran
human	WB	Bioeng Transl Med; Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.; Authors - Fabiao Hu
human	WB	Aging (Albany NY); Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.; Authors - Xinjie Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cassand (Verified Customer) (04-06-2022)

Works good for IHC

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: skin

MMP2 Antibody Immunohistochemistry validation (1:50 dilution) in skin (Cat no:66366-1-Ig)

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA and no antigen retrieval was used.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain at 3dpf

MMP2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain at 3dpf (Cat no:66366-1-Ig)

FH

Ryan (Verified Customer) (01-10-2018)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Retina

MMP2 Antibody Immunohistochemistry, validation (1:100 dilution) in Retina (Cat no:66366-1-Ig)

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