Anticorps Polyclonal de lapin anti-MMP9 (N-terminal)

• Phare
• Validé par KD/KO

MMP9 (N-terminal) Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

1018

Avis (6)

N° de cat : 10375-2-AP

Synonymes

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9, MMP-9, MMP9 (N-terminal)

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Galerie de données de validation

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  WB analysis using 10375-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10375-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat cells using 10375-2-AP

  WB result of MMP9 antibody (10375-2-AP, 1:600) with si-control and si-MMP9 transfected Jurkat cells.

• 

  WB analysis using 10375-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10375-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10375-2-AP (MMP9 (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10375-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP9 (N-terminal) antibody (10375-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10375-2-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human MMP9 (N-terminal) (10375-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HepG2, cellules LNCaP, cellules MDA-MB-231, cellules Raji
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 901 publications below
• IHC: See 141 publications below
• IF: See 62 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

10375-2-AP cible MMP9 (N-terminal) dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, porc, souris, Hamster, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP9 (N-terminal) Protéine recombinante Ag0552
• Nom complet: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Masse moléculaire calculée: 707 aa, 78 kDa
• Poids moléculaire observé: 92 kDa, 67 kDa
• Numéro d’acquisition GenBank: BC006093
• Symbole du gène: MMP9
• Identification du gène (NCBI): 4318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. Matrix metalloproteinase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) (MMP9, synonyms: GELB, CLG4B) degrades collagens type IV and V. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The pro-MMP9 is 92 kDa, and it can be detected a processed form of 68 kDa. This protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
mouse	WB,IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
mouse	WB	Adv Sci (Weinh); Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression; Authors - Rui Zhou
human	WB, IHC	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui
mouse	IF	Acta Pharm Sin B; Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy; Authors - Chang Xiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Siddharth (Verified Customer) (07-18-2023)

Good antibody for IF experiments

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary mesenchymal stromal cells

FH

Christine (Verified Customer) (07-11-2022)

The MMP9 antibody detects nicely a protein with a size of about 100 kD and the blot looks like the blots on the product page

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse macrophage cell line J774

MMP9 (N-terminal) Antibody Western Blot validation (1:1000 dilution) in mouse macrophage cell line J774 (Cat no:10375-2-AP)

FH

Hongxue (Verified Customer) (01-12-2021)

Clear band for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Liver

MMP9 (N-terminal) Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:10375-2-AP)

FH

Kyosuke (Verified Customer) (06-12-2019)

I am working on aneurysm study and use this for IHC staining on mouse aneurysm section. It works very well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: mouse aorta

FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects MMP9 expressing cells in the spinal cord of C57BL/6 EAE mice. The imaging is quiet clear with this antibody.

• Applications: Immunofluorescence,

FH

Lucy (Verified Customer) (03-08-2019)

Reacts mainly to human MMP9 but slightly cross-reactive to human MMP2

• Applications: Western Blot,

MMP9 (N-terminal) Antibody Western Blot, validation ( dilution) in (Cat no:10375-2-AP)

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