Anticorps Polyclonal de lapin anti-MRPS18B

MRPS18B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

53

N° de cat : 16139-1-AP

Synonymes

C6orf14, HSPC183, HumanS18a, MRP S18 2, MRP S18 b, MRPS18 2, Mrps18 b, MRPS18B, PTD017, S18amt, S18mt b

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Galerie de données de validation

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  WB analysis using 16139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16139-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 16139-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16139-1-AP (MRPS18B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16139-1-AP

  IP Result of anti-MRPS18B (IP:16139-1-AP, 4ug; Detection:16139-1-AP 1:1000) with HeLa cells lysate 2800ug.

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  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16139-1-AP (MRPS18B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16139-1-AP (MRPS18B antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules MCF-7, cellules PC-3, cellules SH-SY5Y, tissu cardiaque de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 48 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

16139-1-AP cible MRPS18B dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPS18B Protéine recombinante Ag9198
• Nom complet: mitochondrial ribosomal protein S18B
• Masse moléculaire calculée: 258 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC005373
• Symbole du gène: MRPS18B
• Identification du gène (NCBI): 28973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MRPS18B, also named as S18mt-b, MRP-S18-2 and C6orf14, belongs to the ribosomal protein S18P family. It is a component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. This antibody recognizes specifically the 29 kd MRPS18B protein.

Publications

Species	Application	Title
human	WB	Cell; Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein.; Authors - Ricarda Richter-Dennerlein
	WB,CoIP	Cell Host Microbe; Inducible Germline IgMs Bridge Trypanosome Lytic Factor Assembly and Parasite Recognition.; Authors - Joseph Verdi
human	WB	Cell Metab; The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.; Authors - Antonicka Hana H
human	WB	Nat Struct Mol Biol; Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.; Authors - Xingqian Zhang
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.; Authors - Feilong Meng

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