Anticorps Polyclonal de lapin anti-MX1

• Phare
• Validé par KD/KO

MX1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (6)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

86

Avis (1)

N° de cat : 13750-1-AP

Synonymes

IFI 78K, IFI78, MX, MX1, MxA, Myxovirus resistance protein 1

---

Galerie de données de validation

• 

  WB analysis using 13750-1-AP

  Lysates of A549 cells treated or non-treated with IFN-alpha were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13750-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13750-1-AP (MX1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 cells using 13750-1-AP

  IP result of anti-MX1 (13750-1-AP for IP and Detection) with A549 cell.

• 

  IHC staining of human tonsillitis using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13750-1-AP (MX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 13750-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 13750-1-AP (MX1 Antibody) at dilution of 1:50.

• 

  IF Staining of A549 using 13750-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MX1 antibody (13750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 13750-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MX1 (13750-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A549, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 67 publications below
• IHC: See 9 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

13750-1-AP cible MX1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain, porc, poulet, singe, souris, Hamster, Macaque
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MX1 Protéine recombinante Ag4692
• Nom complet: Myxovirus resistance protein 1
• Masse moléculaire calculée: 662 aa, 76 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC032602
• Symbole du gène: MX1
• Identification du gène (NCBI): 4599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.

Publications

Species	Application	Title
human	WB	Nature; IFITM3 restricts the morbidity and mortality associated with influenza.; Authors - Everitt AR
human	WB	Cell Host Microbe; NRAV, a long noncoding RNA, modulates antiviral responses through suppression of interferon-stimulated gene transcription.; Authors - Jing Ouyang
human		Nat Commun; Large-scale and high-resolution mass spectrometry-based proteomics profiling defines molecular subtypes of esophageal cancer for therapeutic targeting.; Authors - Wei Liu
human	WB	Hepatology; MxA inhibits hepatitis B virus replication by interaction with hepatitis B core antigen.; Authors - Li Ning N
macaque	IHC	Nat Commun; Repeated semen exposure decreases cervicovaginal SIVmac251 infection in rhesus macaques.; Authors - Shaheed A Abdulhaqq
human	WB	J Hepatol; PMID: 25481566; Authors - Shi Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hannah (Verified Customer) (09-21-2020)

Used for western blot on human fibroblasts -/+ interferon treatment, detected single band only in IFN treated samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1/500

MX1 Antibody Western Blot validation (1/500 dilution) in (Cat no:13750-1-AP)

Produits associés

OAS1/3 Polyclonal antibody

14955-1-AP

IFN Gamma Polyclonal antibody

15365-1-AP

IFITM3 Polyclonal antibody

11714-1-AP

IFITM1-Specific Monoclonal antibody

60074-1-Ig

IFITM2-specific Monoclonal antibody

66137-1-Ig

IFITM2/3 Monoclonal antibody

66081-1-Ig

IFITM2 Polyclonal antibody

12769-1-AP