Anticorps Polyclonal de lapin anti-SMMHC

SMMHC Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (1)

N° de cat : 21404-1-AP

Synonymes

MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

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Galerie de données de validation

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  WB analysis of mouse uterus using 21404-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 21404-1-AP (SMMHC antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 21404-1-AP (SMMHC antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21404-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21404-1-AP (SMMHC antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse heart using 21404-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 21404-1-AP (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C2C12 using 21404-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human SMMHC (21404-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu utérin de souris,
• Résultats positifs en IHC: tissu de côlon humain, tissu de muscle squelettique humain, tissu oculaire de rat, tissu oculaire de souris, tissu vésical de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris
• Résultats positifs en cytométrie: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Informations sur le produit

21404-1-AP cible SMMHC dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMMHC Protéine recombinante Ag16114
• Nom complet: myosin, heavy chain 11, smooth muscle
• Masse moléculaire calculée: 1979 aa, 228 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC104906
• Symbole du gène: MYH11
• Identification du gène (NCBI): 4629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Regulated lysosomal exocytosis mediates cancer progression.; Authors - Eda Machado
human	WB	J Clin Invest; UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease.; Authors - Leonardo Elia
mouse	WB	Theranostics; Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.; Authors - Gao-Shan Yang
rat	WB	Acta Physiol (Oxf); Prostacyclin facilitates vascular smooth muscle cell phenotypic transformation via activating TP receptors when IP receptors are deficient.; Authors - Ziqing Li
rat	WB	Biomed Pharmacother; Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ.; Authors - Ji-Ru Zhang
rat	WB	Oxid Med Cell Longev; S-Nitroso-L-Cysteine Ameliorated Pulmonary Hypertension in the MCT-Induced Rats through Anti-ROS and Anti-Inflammatory Pathways.; Authors - Moran Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (07-12-2023)

One of the best smooth muscle cell markers for immunofluorescence!!

• Applications: Immunofluorescence

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