Anticorps Polyclonal de lapin anti-MYL7

MYL7 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (2)

N° de cat : 17283-1-AP

Synonymes

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

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Galerie de données de validation

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  WB analysis using 17283-1-AP

  Mouse heart lysates were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17283-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17283-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 17283-1-AP

  IP Result of anti-MYL7 (IP:17283-1-AP, 4ug; Detection:17283-1-AP 1:700) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse heart using 17283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17283-1-AP (MYL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17283-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17283-1-AP (MYL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 17283-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 17283-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 17283-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.5 ug Anti-Human MYL7 (17283-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris,
• Résultats positifs en cytométrie: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Informations sur le produit

17283-1-AP cible MYL7 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL7 Protéine recombinante Ag11058
• Nom complet: myosin, light chain 7, regulatory
• Masse moléculaire calculée: 175 aa, 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC027915
• Symbole du gène: MYL7
• Identification du gène (NCBI): 58498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

Publications

Species	Application	Title
	IF	J Clin Invest; 1-Deoxynojirimycin promotes cardiac function and rescues mitochondrial cristae in mitochondrial hypertrophic cardiomyopathy; Authors - Qianqian Zhuang
mouse	IF	Nat Commun; High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.; Authors - Yuanbiao Zhao
human	IF	Theranostics; Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction.; Authors - Shi Hua Tan
human	IF	Cardiovasc Res; Angiopoietin-1 enhanced myocyte mitosis, engraftment, and the reparability of hiPSC-CMs for treatment of myocardial infarction.; Authors - Zhonghao Tao
human	WB	Stem Cell Reports; Mitochondrial Dysfunctions Contribute to Hypertrophic Cardiomyopathy in Patient iPSC-Derived Cardiomyocytes with MT-RNR2 Mutation.; Authors - Shishi Li
human	IF	Front Cell Dev Biol; HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.; Authors - Emmi Helle

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Works very well in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Mouse Heart Tissue

MYL7 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:17283-1-AP)

FH

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

MYL7 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:17283-1-AP)

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