Anticorps Polyclonal de lapin anti-Palladin

• Phare
• Validé par KD/KO

Palladin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, COIP, ELISA

Conjugaison

Non conjugué

Publications

69

N° de cat : 10853-1-AP

Synonymes

CGI 151, KIAA0992, palladin, PALLD, PNCA1, Sarcoma antigen NY SAR 77, SIH002

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Galerie de données de validation

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  WB analysis of A549 cells using 10853-1-AP

  A549 cells (shcontrol and shRNA of PALLD) were subjected to SDS PAGE followed by western blot with 10853-1-AP (PALLD antibody) at dilution of 1:2000.

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  WB analysis of HeLa using 10853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10853-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10853-1-AP (Palladin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10853-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10853-1-AP

  IP Result of anti-Palladin (IP:10853-1-AP, 4ug; Detection:10853-1-AP 1:2000) with HeLa cells lysate 1800ug.

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  IP experiment of HEK-293 using 10853-1-AP

  IP Result of anti-Palladin (IP:10853-1-AP, 4ug; Detection:10853-1-AP 1:300) with HEK-293 cells lysate 1500ug.

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10853-1-AP (Palladin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10853-1-AP (Palladin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10853-1-AP (Palladin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10853-1-AP (PALLD,palladin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10853-1-AP

  Immunofluorescent analysis of HeLa cells using 10853-1-AP (Palladin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10853-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PALLD,palladin antibody (10853-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IP: cellules HeLa, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 53 publications below
• IHC: See 12 publications below
• IF: See 43 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10853-1-AP cible Palladin dans les applications de WB, IP, IHC, IF, FC, COIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Palladin Protéine recombinante Ag1288
• Nom complet: palladin, cytoskeletal associated protein
• Masse moléculaire calculée: 1383 aa, 151 kDa
• Poids moléculaire observé: 95 kDa, 140 kDa
• Numéro d’acquisition GenBank: BC013867
• Symbole du gène: PALLD
• Identification du gène (NCBI): 23022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Palladin is an actin associated protein serving as a cytoskeleton scaffold, and actin cross linker, localizing at stress fibers, focal adhesions, and other actin based structures. Palladin exists as multiple isoforms through alternative transcription initiation sites and splicing. There are three major isoforms (200, 140, 90-92 kDa) and multiple minor isoforms. Different palladin isoforms are expressed in a tissue-specific pattern during development and in adult organs: the 200 kDa isoform is predominantly expressed in the heart, skeletal muscle, testis and bone; the 140 kDa isoform is widely expressed with the exception of liver, muscle, and skin; the 90-92 kDa isoform is broadly expressed in embryonic tissues and highly expressed in adult smooth muscle tissues (21455759). Recently overexpression of palladin has been linked to the promoted invasive motility in several types of cancers (18978809, 22291919). The 85-90 kDa palladin isoform has been observed predominantly expressed in pancreatic ductal adenocarcinoma (PDA) while a 65 kDa isoform is expressed in normal pancreas and non-PDA tumors (20436683). This antibody, generated against the fragment 999-1383aa of palladin, recognizes most isoforms of this protein, except isoform 6.

Publications

Species	Application	Title
human	IP	Mol Cell; The actin-bundling protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration.; Authors - Chin Y Rebecca YR
human, mouse	WB,IHC	Nat Commun; Local microRNA delivery targets Palladin and prevents metastatic breast cancer.; Authors - Avital Gilam ; KD Validated
human	WB	Cell Death Differ; lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.; Authors - Hai-Ting Liu
human	IF	J Am Soc Nephrol; The Role of Palladin in Podocytes.; Authors - Nadine Artelt
human	IHC	Cancer Res; Cofilin Drives Cell Invasive and Metastatic Responses to TGF-β in Prostate Cancer.; Authors - Joanne Collazo
human	WB,IHC,IF	Oncogene; Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).; Authors - I García-Palmero ; KD Validated

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