Anticorps Polyclonal de lapin anti-PPP3CA

PPP3CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(18)

Avis (1)

N° de cat : 13422-1-AP

Synonymes

CALNA, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, CALN, CALNA1

---

Galerie de données de validation

• 

  WB analysis of mouse skeletal muscle using 13422-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13422-1-AP (PPP3CA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13422-1-AP

  RAW 264.7 cell lysates were subjected to SDS PAGE followed by western blot with 13422-1-AP (PPP3CA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13422-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13422-1-AP (PPP3CA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13422-1-AP (PPP3CA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13422-1-AP

  IP result of anti-PPP3CA (IP:13422-1-AP, 4ug; Detection:13422-1-AP 1:5000) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of human colon cancer using 13422-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13422-1-AP (PPP3CA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13422-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13422-1-AP (PPP3CA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13422-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13422-1-AP (PPP3CA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13422-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13422-1-AP (PPP3CA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13422-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13422-1-AP (PPP3CA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13422-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP3CA antibody (13422-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 13422-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PPP3CA Polyclonal antibody (13422-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules RAW 264.7, cellules SH-SY5Y, tissu cardiaque de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IF: See 3 publications below

Informations sur le produit

13422-1-AP cible PPP3CA dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP3CA Protéine recombinante Ag4203
• Nom complet: protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC025714
• Symbole du gène: PPP3CA
• Identification du gène (NCBI): 5530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.

Publications

Species	Application	Title
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
mouse	WB	Proc Natl Acad Sci U S A; Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.; Authors - Xiju He
pig	WB	J Agric Food Chem; Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs; Authors - Wenting An
pig	WB	J Cell Physiol; Taurine promotes muscle fiber type transformation through CaN/NFATc1 signaling in porcine myoblasts; Authors - Wenting An
human	WB,IF	Neurobiol Dis; Calcineurin β protects brain after injury by activating the unfolded protein response.; Authors - Yanan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

nuo (Verified Customer) (08-22-2021)

A very good ab to probe PPP3CA by WB. With a high titer.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

PPP3CA Antibody Western Blot validation (1:3000 dilution) in mouse brain lysate (Cat no:13422-1-AP)

{{ptg:RelatedPrimaryAntibodies}}