Anticorps Polyclonal de lapin anti-PKC Iota

• Phare
• Validé par KD/KO

PKC Iota Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 13883-1-AP

Synonymes

PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E

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Galerie de données de validation

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  WB analysis using 13883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13883-1-AP (PKC Iota antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13883-1-AP

  WB result of PKC Iota antibody (13883-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PKC Iota transfected MCF-7 cells.

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  IP experiment of HeLa using 13883-1-AP

  IP result of anti-PKC Iota (IP:13883-1-AP, 4ug; Detection:13883-1-AP 1:2000) with HeLa cells lysate 1720 ug.

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  IHC staining of human breast cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13883-1-AP (PKC iota antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 13883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13883-1-AP (PKC iota antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13883-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKC Iota antibody (13883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 13883-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PKC Iota (13883-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13883-1-AP cible PKC Iota dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Iota Protéine recombinante Ag4990
• Nom complet: protein kinase C, iota
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC022016
• Symbole du gène: PKC Iota
• Identification du gène (NCBI): 5584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

Publications

Species	Application	Title
human	WB,IF	Mol Cancer; circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.; Authors - Wei Gao ; KD Validated
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin ; KD Validated
human,mouse	WB,IHC,IF,CoIP	Mol Med; Paired protein kinases PRKCI-RIPK2 promote pancreatic cancer growth and metastasis via enhancing NF-κB/JNK/ERK phosphorylation; Authors - Juying Jiao
human	WB	Int J Biol Sci; Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.; Authors - Fangfei Li
human	WB,IHC	Onco Targets Ther; Protein kinase C-iota-mediated glycolysis promotes non-small-cell lung cancer progression.; Authors - Liu Liu ; KD Validated
human	WB,IHC,IP	Antioxidants (Basel); PKCiota Inhibits the Ferroptosis of Esophageal Cancer Cells via Suppressing USP14-Mediated Autophagic Degradation of GPX4; Authors - Hao Tao ; KD Validated

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