Anticorps Polyclonal de lapin anti-PSMA8

• Phare
• Validé par KD/KO

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 14022-1-AP

Synonymes

Alpha4s, Proteasome alpha 4 subunit, Proteasome subunit alpha-type 7-like, Proteasome subunit alpha-type 8, PSMA7L

---

Galerie de données de validation

• 

  WB analysis using 14022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14022-1-AP

  WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

• 

  WB analysis of K-562 using 14022-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14022-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14022-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 14022-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14022-1-AP cible PSMA8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA8 Protéine recombinante Ag5138
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 8
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC042820
• Symbole du gène: PSMA8
• Identification du gène (NCBI): 143471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis.; Authors - Zhiming Li
mouse	IF	PLoS Genet; The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.; Authors - Laura Gómez-H
mouse	WB	Sci China Life Sci; Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice; Authors - Yiling Zhou
		Zool Res; Both 20S and 19S proteasome components are essential for meiosis in male mice; Authors - Ting-Ting Han

{{ptg:RelatedPrimaryAntibodies}}