Anticorps Polyclonal de lapin anti-PSMD4

• Phare
• Validé par KD/KO

PSMD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 14899-1-AP

Synonymes

26S proteasome non-ATPase regulatory subunit 4, AF, ASF, MCB1, Multiubiquitin chain-binding protein

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Galerie de données de validation

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  WB analysis using 14899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14899-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14899-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14899-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14899-1-AP (PSMD4 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 14899-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 14899-1-AP (PSMD4 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 14899-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMD4 antibody (14899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 14899-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PSMD4 (14899-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, cellules NIH/3T3, cellules Raji, cellules RAW264.7, tissu splénique de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14899-1-AP cible PSMD4 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMD4 Protéine recombinante Ag6691
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC002365
• Symbole du gène: PSMD4
• Identification du gène (NCBI): 5710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Publications

Species	Application	Title
mouse	WB,IF	Nat Neurosci; Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.; Authors - Rossella Avagliano Trezza
mouse	IF	J Cell Biol; The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.; Authors - Christoph Gerhardt
human	WB	Cancer Res; The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.; Authors - Xiuxia Liu
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	WB	J Cell Sci; S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.; Authors - Zheng Wang
human	WB	Mol Cell Proteomics; Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.; Authors - Tatjana Goebel

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