Anticorps Polyclonal de lapin anti-PTH2R

PTH2R Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 14166-1-AP

Synonymes

parathyroid hormone 2 receptor, PTH2 receptor, PTH2R, PTHR2

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Galerie de données de validation

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  WB analysis of L02 using 14166-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14166-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14166-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14166-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14166-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14166-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14166-1-AP (PTH2R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 14166-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 14166-1-AP (PTH2R antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of BxPC-3 using 14166-1-AP

  1X10^6 BxPC-3 cells were stained with 0.2ug PTH2R antibody (14166-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules L02, cellules BxPC-3, tissu hépatique de souris, tissu pancréatique de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14166-1-AP cible PTH2R dans les applications de WB, IHC, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTH2R Protéine recombinante Ag5364
• Nom complet: parathyroid hormone 2 receptor
• Masse moléculaire calculée: 550 aa, 62 kDa
• Poids moléculaire observé: 62-66 kDa
• Numéro d’acquisition GenBank: BC036811
• Symbole du gène: PTH2R
• Identification du gène (NCBI): 5746
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.