• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-RAB7A

RAB7A Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 55469-1-AP

Synonymes

PRO2706, RAB7, RAB7A, Ras related protein Rab 7a



Applications testées

Résultats positifs en WBcellules L-929, cellules A431, cellules C6, cellules NIH/3T3, cellules RAW 264.7, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
Résultats positifs en IHCtissu rénal humain, tissu de muscle squelettique humain, tissu d'estomac humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en cytométriecellules A431,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochimie (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

55469-1-AP cible RAB7A dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, poisson-zèbre, porc, singe, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet RAB7A, member RAS oncogene family
Masse moléculaire calculée 23 kDa
Poids moléculaire observé 23 kDa
Numéro d’acquisition GenBankNM_004637
Symbole du gène RAB7A
Identification du gène (NCBI) 7879
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB7A, also named as RAB7, belongs to the small GTPase superfamily and Rab family. It is involved in late endocytic transport. RAB7A contributes to the maturation of phagosomes (acidification). Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B). This antibody is specific to RAB7A.

Protocole

Product Specific Protocols
WB protocol for RAB7A antibody 55469-1-APDownload protocol
IHC protocol for RAB7A antibody 55469-1-APDownload protocol
IP protocol for RAB7A antibody 55469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
humanWB

Nat Commun

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Authors - Shen Zhang
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
Zebrafish,monkey

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun

Nat Commun

CHML promotes liver cancer metastasis by facilitating Rab14 recycle.

Authors - Tian-Wei Chen
humanWB

Autophagy

Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress

Authors - Jia-min Yan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sophy (Verified Customer) (03-07-2023)

The antibody worked well in western with 1:1000 dilution. The signal was somewhat weak but that could also be due to the cell types that were used.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NSCLC cells
FH

Malgorzata (Verified Customer) (08-17-2022)

The antibody worked on my positive control: Lysate of THP1 cell line. Nevertheless, for my samples it gave strong nonspecific signal in high MW

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: THP-1
RAB7A Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:55469-1-AP)
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by RAB7A Polyclonal antibody at dilution of 1:50 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NSC-34 cells
FH

Laura (Verified Customer) (01-15-2020)

Works well for Western Blot at 1:1000 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

Aamir (Verified Customer) (01-08-2020)

WB at 1 in 500IF at 1 in 250

  • Applications: Western Blot, Immunohistochemistry,
  • Cell Tissue Type: HEKs
FH

Benjamin (Verified Customer) (12-10-2019)

Has worked well multiple times with dilution of 1:1000 with the RAB7A antibody. I always incubate at 4 degrees overnight in 3% milk TBST solution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293 Lysate
RAB7A Antibody Western Blot, validation (1:1000 dilution) in HEK293 Lysate (Cat no:55469-1-AP)