Anticorps Monoclonal anti-RALBP1

RALBP1 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B2F5

N° de cat : 67359-1-Ig

Synonymes

DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 67359-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67359-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67359-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67359-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67359-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67359-1-Ig (RALBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HEK-293 using 67359-1-Ig

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug RALBP1 Monoclonal antibody (67359-1-Ig, Clone:1B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules Jurkat, cellules LNCaP, cellules MCF-7
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67359-1-Ig cible RALBP1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RALBP1 Protéine recombinante Ag28618
• Nom complet: ralA binding protein 1
• Masse moléculaire calculée: 655 aa, 76 kDa
• Poids moléculaire observé: 95 kDa
• Numéro d’acquisition GenBank: BC013126
• Symbole du gène: RALBP1
• Identification du gène (NCBI): 10928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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