Anticorps Polyclonal de lapin anti-RBPMS

• Phare
• Validé par KD/KO

RBPMS Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(36)

Avis (2)

N° de cat : 15187-1-AP

Synonymes

Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

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Galerie de données de validation

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  WB analysis using 15187-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15187-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15187-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15187-1-AP (RBPMS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15187-1-AP

  IP result of anti-RBPMS (IP:15187-1-AP, 4ug; Detection:15187-1-AP 1:400) with mouse heart tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 15187-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15187-1-AP (RBPMS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 15187-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15187-1-AP (RBPMS antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of rat eye using 15187-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using RBPMS antibody (15187-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15187-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (15187-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 15187-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBPMS (15187-1-AP) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu de côlon de rat, tissu de côlon de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de rat,
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 26 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

15187-1-AP cible RBPMS dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBPMS Protéine recombinante Ag7256
• Nom complet: RNA binding protein with multiple splicing
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC003608
• Symbole du gène: RBPMS
• Identification du gène (NCBI): 11030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.

Publications

Species	Application	Title
mouse	IF	Cell; Perivascular neurons instruct 3D vascular lattice formation via neurovascular contact; Authors - Kenichi Toma
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	IF	Cell Rep; Comparative transcriptomic profiling reveals a role for Olig1 in promoting axon regeneration; Authors - Xiu-Qing Fu
human	WB,IP	Cell Rep; Pan-cancer tRNA-derived fragment CAT1 coordinates RBPMS to stabilize NOTCH2 mRNA to promote tumorigenesis; Authors - Mengqian Yu
mouse	IHC	Cell Rep; Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina.; Authors - Thanh Hoang
mouse	IF	Sci Rep; Develop an efficient and specific AAV-based labeling system for Muller glia in mice; Authors - Yanxia Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christoffer (Verified Customer) (03-03-2025)

Does not stain specific, lots of background.

• Applications: Immunohistochemistry, Immunofluorescence
• Cell Tissue Type: Adult mouse retina embedded in paraffin.

FH

Tom (Verified Customer) (07-26-2022)

This antibody works great for western blot. I had a very specific band at the expected MW of RBPMS. Highly recommended!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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