Anticorps Polyclonal de lapin anti-RGS5

RGS5 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 11590-1-AP

Synonymes

MST092, MST106, MST129, MSTP032, MSTP092, MSTP106, MSTP129, RGS5

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Galerie de données de validation

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  WB analysis using 11590-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11590-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11590-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11590-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11590-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11590-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11590-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 11590-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11590-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11590-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RGS5 antibody (11590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11590-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RGS5 (11590-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules MCF-7, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de rat, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Informations sur le produit

11590-1-AP cible RGS5 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS5 Protéine recombinante Ag2196
• Nom complet: regulator of G-protein signaling 5
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC030059
• Symbole du gène: RGS5
• Identification du gène (NCBI): 8490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)

Publications

Species	Application	Title
mouse	WB,IHC	Cell; Beta-Arrestin1 Prevents Preeclampsia by Downregulation of Mechanosensitive AT1-B2 Receptor Heteromers.; Authors - Ursula Quitterer
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	IF	Adv Sci (Weinh); Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer; Authors - Ting Liang
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
mouse	WB	Arterioscler Thromb Vasc Biol; HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.; Authors - Shanshan Liu
mouse	WB,IF	PLoS One; A regulator of G protein signaling 5 marked subpopulation of vascular smooth muscle cells is lost during vascular disease.; Authors - Ya-Kun Gao