Anticorps Polyclonal de lapin anti-RNH1

• Phare
• Validé par KD/KO

RNH1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10345-1-AP

Synonymes

Placental RNase inhibitor, PRI, RAI, Ribonuclease inhibitor, RNH, RNH1

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Galerie de données de validation

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 10345-1-AP

  WB result of RNH1 antibody (10345-1-AP, 1:2500) with si-control and si-RNH1 transfected HeLa cells.

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  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10345-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10345-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10345-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10345-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10345-1-AP (RNH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10345-1-AP

  IP Result of anti-RNH1 (IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IP experiment of HEK-293T using 10345-1-AP

  IP result of anti-RNH1(IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with HEK-293T cells lysate 2040 ug.

• 

  IP experiment of mouse liver using 10345-1-AP

  IP result of anti-RNH1(IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

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  IP experiment of rat liver using 10345-1-AP

  IP result of anti-RNH1(IP:10345-1-AP, 4ug; Detection:10345-1-AP 1:8000) with rat liver tissue lysate 5520 ug.

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10345-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10345-1-AP (RNH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10345-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RNH1 antibody (10345-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 10345-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RNH1 (10345-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules L02, tissu cardiaque humain, tissu cérébral humain, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IP: tissu hépatique de souris, cellules HEK-293T, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10345-1-AP cible RNH1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNH1 Protéine recombinante Ag0350
• Nom complet: ribonuclease/angiogenin inhibitor 1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC000677
• Symbole du gène: RNH1
• Identification du gène (NCBI): 6050
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonuclease/ angiogenin inhibitor (RNH, synonyms: RAI, MGC4569, MGC18200, MGC54054) is a protein that binds tightly to ribonucleases in cells and may be essential in the control of mRNA degradation and gene expression. The human RNH gene has been regionally localized to chromosome band 11p15 by in situ hybridization.

Publications

Species	Application	Title
mouse	WB	EMBO J; Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.; Authors - Rongpan Bai
human	WB	J Cell Biol; Angiogenin cleaves tRNA and promotes stress-induced translational repression.; Authors - Yamasaki Satoshi S ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.; Authors - Shozo Honda
human	WB	Int J Mol Sci; RTCB Complex Regulates Stress-Induced tRNA Cleavage; Authors - Yasutoshi Akiyama
human	IHC	J Proteome Res; Post-translational modifications and protein-specific isoforms in endometriosis revealed by 2D DIGE.; Authors - Stephens Andrew N AN
mouse	WB	J Biol Chem; Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress.; Authors - Saikia Mridusmita M